English to Russian Medical Chemistry; Chem Sci/Eng

An exemplary protocol for the peptide synthesis process using SPPS comprises the following. 1) Add DMF to a vessel containing link amide MBHA resin and expand for 2 hours ***(sub:*** 0.68 mmol/g, 1.0 mmol, 1.47 g or 5 mmol, 7.35 g, sub: 0.68 mmol/g). 2) After adding 20% piperidine/DMF, mix for 30 minutes. 3) After removing the solvent of 1)-2), wash using DMF (30 seconds x 5 times). 4) Add the reactant (one of the reactants #1 to #5) and mix for 30 seconds, then add the coupling reagent (one of the coupling reagents #1 to #5) corresponding to the reactant, and carry out nitrogen bubbling for 1 hour. 5) After adding 20% piperidine/DMF, mix for 30 minutes.